PCR-Assisted Contig Extension: Stepwise Strategy for Bacterial Genome Closure
نویسندگان
چکیده
منابع مشابه
PCR-assisted contig extension: stepwise strategy for bacterial genome closure.
Finishing is rate limiting for genome projects, and improvements in the efficiency of complete genome-sequence compilation will require improved protocols for gap closure. Here we report a novel approach for extending shotgun contigs and closing gaps that we termed PCR-assisted contig extension (PACE). PACE depends on the capture of rare mismatched interactions that occur between arbitrary prim...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03343dd05